Damage is only affected by size difference in that AP is reduced more for trampling larger opponents, so the total damage doled out by a single trample sequence will be less if the opponents are large.
Damage on a single successfully trampled unit is only determined by the size of the trampler, not the size difference.
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Yes, but he AP value is very important, and what it actually works out to is more damage on smaller creatures are you rush them, with relative size pretty critical.